Journal: Antioxidants

Article Title: Ethnomedicinal Plants with Protective Effects against Beta-Amyloid Peptide (Aβ)1-42 Indicate Therapeutic Potential in a New In Vivo Model of Alzheimer’s Disease

doi: 10.3390/antiox11101865

Figure Lengend Snippet: β-secretase (BACE-1) inhibitory activity of plant extracts. ( A ) Plant extract ( n = 18) concentrations were initially screened at a concentration of 500 µg/mL; ( B – E ) the concentration-dependent activities (15.6–1000 µg/mL) were then determined for four selected phytoextracts, i.e., S. officinalis, J. regia, E. cardamomum , and F. thunbergii. The inhibitory activity was determined by using a standardised BACE-1 Activity Detection Kit, with KF-14 (25 µM) used as a positive control. * p < 0.05, ** p < 0.01, and *** p < 0.001 compared with negative control (vehicle control, i.e., 1% DMSO in ddH 2 O). ns (not significant).

Article Snippet: A known peptide (Lys-Thr-Glu-Glu-Ile-Ser-Glu-Val-Asn-Sta-Val-Ala-Glu-Phe) inhibitor of BACE-1 (KF-14; 25 µM) was custom synthesised (GL Biochem, China) and used as the PC (solution of the buffer with the BACE-1 substrate and BACE-1/KF-14 enzyme mixture).

Techniques: Activity Assay, Concentration Assay, Positive Control, Negative Control

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease

doi: 10.3892/etm.2015.2391

Figure Lengend Snippet: Comparison of the results of BACE1 detection by ELISA (mean ± standard deviation).

Article Snippet: The proteins were extracted, subjected to SDS-PAGE and then transferred to a nitrocellulose membrane, where they were blocked with 5% bovine serum albumin in phosphate-buffered saline Tween-20 solution for 30 min. Rabbit anti-human BACE1 polyclonal antibodies (#L4391; Wuhan Boster Biological Technology, Ltd., Wuhan, China) diluted with Tris-buffered saline (TBS) and Tween 20 (TBST; 1:2,000) were added, and the membrane was incubated overnight at 4°C.

Techniques: Comparison, Enzyme-linked Immunosorbent Assay, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease

doi: 10.3892/etm.2015.2391

Figure Lengend Snippet: Comparison of the detection results of BACE1 by western blot assay (mean ± standard deviation).

Article Snippet: The proteins were extracted, subjected to SDS-PAGE and then transferred to a nitrocellulose membrane, where they were blocked with 5% bovine serum albumin in phosphate-buffered saline Tween-20 solution for 30 min. Rabbit anti-human BACE1 polyclonal antibodies (#L4391; Wuhan Boster Biological Technology, Ltd., Wuhan, China) diluted with Tris-buffered saline (TBS) and Tween 20 (TBST; 1:2,000) were added, and the membrane was incubated overnight at 4°C.

Techniques: Comparison, Western Blot, Control

Journal: Experimental and Therapeutic Medicine

Article Title: Expression of β-site APP-cleaving enzyme 1 in the hippocampal tissue of an insulin-resistant rat model of Alzheimer's disease

doi: 10.3892/etm.2015.2391

Figure Lengend Snippet: Comparison of the results of BACE1 detection by RT-qPCR (mean ± standard deviation).

Article Snippet: The proteins were extracted, subjected to SDS-PAGE and then transferred to a nitrocellulose membrane, where they were blocked with 5% bovine serum albumin in phosphate-buffered saline Tween-20 solution for 30 min. Rabbit anti-human BACE1 polyclonal antibodies (#L4391; Wuhan Boster Biological Technology, Ltd., Wuhan, China) diluted with Tris-buffered saline (TBS) and Tween 20 (TBST; 1:2,000) were added, and the membrane was incubated overnight at 4°C.

Techniques: Comparison, Control

Journal: Human Molecular Genetics

Article Title: Mitochondria-targeted catalase reduces abnormal APP processing, amyloid ? production and BACE1 in a mouse model of Alzheimer's disease: implications for neuroprotection and lifespan extension

doi: 10.1093/hmg/dds128

Figure Lengend Snippet: Aβ levels using sandwich enzyme linked immuno sorbant assay (ELISA) in brain sections from 6-, 12- and 24-month-old AβPP and MCAT/AβPP mice. Aβ40 and Aβ42 were quantified using sandwich ELISA and expressed as pg Aβ/mg protein. (A) Aβ40 levels in two transgenic lines at the three ages; (B) Aβ42 levels in two transgenic lines at the three ages; (C) Aβ40 levels in the MCAT/AβPP and AβPP mice and (D) Aβ42 levels in the MCAT/AβPP and AβPP mice.

Article Snippet: Details of the antibodies are given in Table . table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody Secondary antibody Species and dilution Purchased from Dilution, Alexa dye Purchased from Anti-Aβ (6E10) Mouse monoclonal 1:200 Covance, San Diego, CA, USA Goat anti-mouse biotin 1:400, ABC, TSA-Alexa594 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA Anti-Aβ42 (AB05) Mouse monoclonal (ready to use from the kit) Wako Pure Chemicals, Japan Goat anti-mouse biotin, ABC solution, TSA-Alexa594 Wako Pure Chemicals, Japan Anti-Aβ40 (BA27) Mouse monoclonal (ready to use from the kit) Wako Pure Chemicals, Japan Goat anti-mouse biotin, ABC solution, TSA-Alexa594 Wako Pure Chemicals, Japan Catalase Rabbit polyclonal 1:200 Abcam, Cambridge, MA, USA Goat anti-rabbit biotin 1:400, ABC, TSA-Alexa 488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA BACE1 Rabbit polyclonal 1:150 Calbiochem, San Diego, CA, USA Goat anti-rabbit biotin 1:400, ABC,TSA-Alexa488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA 8-OHdG Goat polyclonal, 1:200 Neuromics, Edina, MN, USA Horse anti-goat biotin, 1:400, ABC,TSA-Alexa488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence using brain sections from transgenic mice and control WT mice We quantified Aβ40- and Aβ42-specific deposits.

Techniques: Enzyme-linked Immunosorbent Assay, Sandwich ELISA, Transgenic Assay

Journal: Human Molecular Genetics

Article Title: Mitochondria-targeted catalase reduces abnormal APP processing, amyloid ? production and BACE1 in a mouse model of Alzheimer's disease: implications for neuroprotection and lifespan extension

doi: 10.1093/hmg/dds128

Figure Lengend Snippet: Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence using brain sections from transgenic mice and control WT mice

Article Snippet: Details of the antibodies are given in Table . table ft1 table-wrap mode="anchored" t5 Table 6. caption a7 Marker Primary antibody Secondary antibody Species and dilution Purchased from Dilution, Alexa dye Purchased from Anti-Aβ (6E10) Mouse monoclonal 1:200 Covance, San Diego, CA, USA Goat anti-mouse biotin 1:400, ABC, TSA-Alexa594 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA Anti-Aβ42 (AB05) Mouse monoclonal (ready to use from the kit) Wako Pure Chemicals, Japan Goat anti-mouse biotin, ABC solution, TSA-Alexa594 Wako Pure Chemicals, Japan Anti-Aβ40 (BA27) Mouse monoclonal (ready to use from the kit) Wako Pure Chemicals, Japan Goat anti-mouse biotin, ABC solution, TSA-Alexa594 Wako Pure Chemicals, Japan Catalase Rabbit polyclonal 1:200 Abcam, Cambridge, MA, USA Goat anti-rabbit biotin 1:400, ABC, TSA-Alexa 488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA BACE1 Rabbit polyclonal 1:150 Calbiochem, San Diego, CA, USA Goat anti-rabbit biotin 1:400, ABC,TSA-Alexa488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA 8-OHdG Goat polyclonal, 1:200 Neuromics, Edina, MN, USA Horse anti-goat biotin, 1:400, ABC,TSA-Alexa488 KPL, Gaithersburg, MD, USA VECTOR Laboratories, Burlingame, CA, USA Molecular Probes, Eugene, OR, USA Open in a separate window Summary of antibody dilutions and conditions used in the immunohistochemistry/immunofluorescence using brain sections from transgenic mice and control WT mice We quantified Aβ40- and Aβ42-specific deposits.

Techniques: Immunohistochemistry, Transgenic Assay, Plasmid Preparation